anti-5-Methylcytosine, Rabbit Monoclonal (RM231)

5-Methylcytosine is a methylated form of the DNA base cytosine that regulates gene transcription and is involved in epigenetics. It is one of the best known epigentic modifications. This methylation typically occurs at cytosine in CpG dinucleotides in vertebrates. When cytosine is methylated, the DNA maintains the same sequence, but the expression of methylated genes can be altered. 5-Methylcytosine is a very important repressor of transcription in the genome. When present in promoters, 5-Methylcytosine is associated with stable, long-term transcriptional silencing. This may occur by either blocking positive transcription factors, or promoting the binding of negative ones.

Product Details
Synonyms 5-mC
Product Type Recombinant Antibody
Clone RM231
Isotype Rabbit IgG
Source/Host Rabbit
Immunogen/Antigen BSA-conjugated 5-methylcytosine.

DotBlot: 0.5 µg/ml-2 µg/ml
ELISA: 0.1 µg/ml-1 µg/ml
Immunocytochemistry (ICC): 0.5 µg/ml-2 µg/ml

Crossreactivity All Vertebrates

This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.

Purity Protein A purified.
Purity Detail Protein A affinity purified from an animal origin-free culture supernatant.
Concentration 1 mg/ml
Formulation Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Isotype Negative Control

Rabbit IgG

Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Accession Number N/A
Declaration Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping BLUE ICE
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.