anti-5-Hydroxymethylcytosine, Rabbit Monoclonal (RM236)

5-Hydroxymethylcytosine (5hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC). It not only marks active demethylation but also serves as a relatively stable DNA mark that plays distinct epigenetic roles. It is potentially important in epigenetics, because the hydroxymethyl group on the cytosine can possibly switch a gene on and off. 5hmC is abundantly expressed, but the levels vary significantly depending on the cell type. The highest levels are found in neuronal cells of the central nervous system.

Product Details
Synonyms 5-hmC
Product Type Recombinant Antibody
Clone RM236
Isotype Rabbit IgG
Source/Host Rabbit
Immunogen/Antigen BSA-conjugated 5-hydroxymethylcytosine.

DotBlot: 0.2 µg/ml-1 µg/ml
ELISA: 0.1 µg/ml-1 µg/ml
Immunocytochemistry (IHC): 0.1 µg/ml-1 µg/ml

Crossreactivity All Vertebrates

This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

Purity Protein A purified.
Purity Detail Protein A affinity purified from an animal origin-free culture supernatant.
Concentration 1 mg/ml
Formulation Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Isotype Negative Control

Rabbit IgG

Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Accession Number N/A
Declaration Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping BLUE ICE
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.