anti-β-Tubulin, mAb (rec.) (S11B)

anti-β-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Product References
Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003)

Product Details
Synonyms Tubulin β Chain, Tubulin β-5 chain
Product Type Recombinant Antibody
Clone S11B
Isotype Human IgG2λ
Source/Host Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen Full length tubulin from pig brain.

Immunocytochemistry: (1:100)
Western blot: (1:1000)

Crossreactivity Drosophila

Recognizes human, mouse, rat, pig, drosophila and monkey β-tubulin.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Other Product Data

anti-β-Tubulin, monoclonal antibody (recombinant) (S11B) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 month after receipt when stored at +4°C.
Stable for at least 1 year after receipt when stored at -20°C.