anti-α-Tubulin, mAb (rec.) (F2C)

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.

Product References
Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003)
Characterization of single chain antibody targets through yeast two hybrid: O. Vielemeyer, et al; BMC Biotechnol. 10, 59 (2010)
AIM2 inflammasome is activated by pharmacological disruption of nuclear envelope integrity: A. Di Micco, et al.; PNAS 113, e4671 (2016)
Pharmacological eEF2K activation promotes cell death and inhibits cancer progression: A. De Gassart, et al.; EMBO Reports 17, 1471 (2016)
Tumor-induced reshuffling of lipid composition on the endoplasmic reticulum membrane sustains macrophage survival and pro-tumorigenic activity: G. Di Conza, et al.; Nat. Immunol. 22, 1403 (2021)
NLRP3 leucine-rich repeats control induced and spontaneous inflammasome activation in cryopyrin-associated periodic syndrome: K. Theodoropoulou, et al.; J. Allerg. Clin. Immunol. ahead of print (2022)
The protease DDI2 regulates NRF1 activation in response to cadmium toxicity: S.T. Ribeiro, et al.; iScience 25, 105227 (2022)

Product Details
Product Type Recombinant Antibody
Clone F2C
Isotype Human IgG2λ
Source/Host Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen Bovine brain tubulin.

Immunocytochemistry: (1:1'000)
Western Blot: (1:1’000)

Crossreactivity Bovine

Recognizes mouse, bovine and human α-tubulin.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Other Product Data

anti-α-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.

anti-α-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 month after receipt when stored at +4°C.
Stable for at least 1 year after receipt when stored at -20°C.