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InVivoKines

InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

Product Details
Synonyms IL-12 (mouse):Fc Knobs-into-Holes (human) (rec.); Interleukin-12 Subunit α:Interleukin-12 Subunit β; CLMF 35kDa Subunit:CLMF 40kDa Subunit; IL-12 Subunit p35:IL-12 Subunit
Product Type Protein
Properties
Source/Host CHO cells. Produced using animal component-free medium.
Sequence

IL-12A/p35 (aa 23-215) (mouse):Fc Knobs and IL-12B/p40 (aa 23-335) (mouse):Fc Holes form the IL-12 (mouse):Fc-KIH (human) using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).

Crossreactivity Mouse
Specificity

Binds to the mouse IL-12Rβ1 protein. Fc (LALA-PG)-KIH (human) does not bind to FcRs.

MW ~60kDa and 75 kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration 1mg/ml after reconstitution.
Reconstitution Reconstitute with 50μl sterile water.
Accession Number P43431, P43432
Formulation Lyophilized. Contains PBS
Protein Negative Control

Fc-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0015)

Other Product Data

UniProt link P43431:  IL-12A (mouse)

UniProt link P43432: IL-12B (mouse)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF