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anti-β-Pix (mouse), pAb

Description
β-PIX acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. It interacts with PAK kinases through the SH3 domain. It may function as a positive regulator of apoptosis and is involved in cell migration. It is expressed with high levels in the central nervous system.

Product References
Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion: W.K. Oh, et al.; BBRC 235, 794 (1997)
betaPix-b(L), a novel isoform of betaPix, is generated by alternative translation: S. Rhee, et al.; BBRC 318, 415 (2004)
Enhanced cytotoxic effect of camptothecin nanosponges in anaplastic thyroid cancer cells in vitro and in vivo on orthotopic xenograft tumors: C.L. Gigliotti, et al.; Drug Deliv. 24, 670 (2017)
Differential Modulation of Human M1 and M2 Macrophage Activity by ICOS‐Mediated ICOSL Triggering: C.L. Gigliotti, et al.; Int. J. Mol. Sci. 24, 2953 (2023)

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Product Details
Synonyms ARHGEF7; Rho Guanine Nucleotide Exchange Factor 7; PAK-interacting Exchange Factor β; p85SPR; COOL-1
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Recombinant β-Pix SH3 domain.
Application

ELISA: (direct and indirect: 1:5'000-1:10'000)
Immunocytochemistry: (1:1'000)
Western Blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.

Crossreactivity Human
Mouse
Rat
Specificity

Recognizes the multiple splice variants of human, mouse and rat β-Pix a, b, bL, c and d-isoforms. Cross-reacts with human and rat β-Pix.
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control

Rabbit IgG

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.

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