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anti-Acetyl-alpha-Tubulin (Lys40), Rabbit Monoclonal (RM318)

Description
Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of α- and β-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different α- and β-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on α- and β-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Tubulin acetylation was discovered on K40 of flagellar α-tubulin in Chlamydomonas reinhardtii and is generally enriched on stable microtubules in cells. It is located on the microtubule lumenal surface. As a result of its localization at the inner face of microtubules, K40 acetylation might rather affect the binding of microtubule inner proteins, a poorly characterized family of proteins. Functional experiments in cells have further suggested that K40 acetylation regulates intracellular transport by regulating the traffic of kinesin motors probably by indirect mechanisms. Acetyltransferase α-Tat1 (or Mec-17) specifically acetylate α-tubulin K40. Acetylation of tubulin by α-Tat1 accumulates selectively in stable, long-lived microtubules thus explaining the link between this posttranslational modication and stable microtubules in cells. However, the direct cellular function of K40 acetylation on microtubules is still unclear.

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Product Details
Synonyms alpha-Tubulin K40ac
Product Type Recombinant Antibody
Properties
Clone RM318
Isotype Rabbit IgG
Source/Host Rabbit
Immunogen/Antigen An acetyl-peptide corresponding to Acetyl- alpha-Tubulin (Lys40).
Application

Immunocytochemistry (ICC): 1:1000-1:5000 dilution
Western Blot (WB): 1:1000-1:2000 dilution
Crossreactivity All Vertebrates
Specificity

This antibody reacts to alpha-Tubulin acetylated at Lysine 40. No cross reactivity to non-acetylated alpha-Tubulin at Lysine 40. This antibody may also react to mouse or rat Acetyl- alpha-Tubulin at Lysine 40, as predicted by immunogen homology.
Purity Protein A purified.
Purity Detail Protein A affinity purified from an animal origin-free culture supernatant.
Concentration N/A
Formulation Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Isotype Negative Control

Rabbit IgG

Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Accession Number P68363
Declaration Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping BLUE ICE
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.

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