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Measuring Inflammasome Activation by Asc Speck Formation


AdipoGen Life Sciences' anti-Asc, pAb (AL177) is the Standard antibody to detect Asc by Western blotting. It can also be used to measure inflammasome activation in vivo, detecting Asc speck formation by Immunofluorescence and by Flow Cytometry.

See our Website for Protocols and Instructions for Flow Cytometry and Immunofluorescence applications using AL177.

More Information
Product Details
Synonyms Pycard; Target of Methylation-induced Silencing 1; TMS1; Caspase Recruitment Domain-containing Protein 5; Apoptosis-associated Speck-like Protein Containing CARD; CARD5
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Synthetic peptide corresponding to aa at the N-terminal human Asc.
Label/Conjugates ATTO 647N
Application

Flow Cytometry (1:1000; Reference 22)
Immunocytochemistry: (1:200)
Immunohistochemistry: (1:500; paraffin sections)

The antibody AL177 can be used to measure inflammasome activation in vivo, detecting Asc speck formation by immunofluorescence and Flow Cytometry.

Crossreactivity Human
Mouse
Specificity

Recognizes human and mouse Asc.

Purity Detail Epitope-affinity purified.
Concentration 0.5mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Use/Stability Keep conjugated formats at +4°C.
Stable for at least 1 year after receipt when stored at +4°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF